Journal: Molecular Therapy. Methods & Clinical Development
Article Title: SERCA2a overexpression improves muscle function in a canine Duchenne muscular dystrophy model
doi: 10.1016/j.omtm.2024.101268
Figure Lengend Snippet: Evaluation of the T cell response in AAV.SERCA2a-injected and contralateral excipient-injected muscle of affected dogs Representative photomicrographs of H&E staining, CD8 (a marker for cytotoxic T cells), CD4 (a marker for helper T cells), and FoxP3 (a marker for regulatory T cells) immunohistochemistry staining and FLAG tag immunofluorescence staining. Right-column photomicrographs are high-magnification images of the boxed areas in the corresponding middle-column photomicrographs. Filled arrowhead, CD8+ T cells; open arrowhead, CD4+ T cells; arrow, FoxP3+ nuclei.
Article Snippet: Immunohistochemistry staining was used to detect CD4+ T cells, CD8+ T cells, and FoxP3+ nuclei, according to our established protocols., Specifically, CD4+ T cells were detected using a rat monoclonal IgG2a antibody against canine CD4 (1:1000; Bio-Rad, cat. no. MCA1038GA, clone YKIX 302.9).
Techniques: Injection, Staining, Marker, Immunohistochemistry, FLAG-tag, Immunofluorescence